Scientific documentation — UFP500 technology platform

The science behind
what performs.

At particle sizes between one and five microns, the rules governing bioavailability, absorption kinetics, and ingredient interaction change fundamentally. UFP500 is a processing platform built around that change — a measurable, reproducible physical reality.

Platform specification — v2.1
Target particle range 1–5 μm
Process temperature Ambient / cold
Solvent use None
Enzymatic integrity Preserved
Output form Dry powder
Processing unit UFP500 platform
Processing environment Dedicated R&D facility
Operator BioFund Ventures
The physics

Why particle size is not a detail.

Conventional milling and grinding reduces particle size in the 100–500 micron range — enough to improve texture and homogeneity, but not enough to meaningfully alter the biophysics of absorption. The shift in behaviour begins somewhere between 10 and 20 microns, and becomes pronounced below 5 microns.

At 1–5 microns, three things change simultaneously:

  • Surface-area-to-volume ratio increases dramatically — a 5-micron particle has roughly 100× the relative surface area of a 500-micron particle, which directly governs dissolution rate and enzymatic contact.
  • Mucosal penetration dynamics shift — particles below 5 microns can interact with intestinal epithelium at a fundamentally different level than larger particles, even before any lipid encapsulation or permeability enhancer is involved.
  • Phagocytic uptake becomes relevant — macrophages and dendritic cells in the gut-associated lymphoid tissue are particle-size-sensitive in the 1–5 micron range.

These are not hypotheses. They are well-documented in the pharmaceutical literature on fine-particle APIs, and in the food science literature on nanoparticle and microparticle absorption. UFP500 applies the same physics to botanical and functional ingredient matrices — a category the pharma and food science communities have largely approached with far less scientific discipline than synthetic actives.

The challenge is not the theory. It is achieving this particle range reliably, without heat, without solvents, and without destroying the very bioactive structures you are trying to preserve. That is where UFP500 operates.

→ Explore the Literature section for full references and supporting evidence
Comparative particle size — μm scale
UFP500 output 1–5 μm
Fine-particle API (pharma) 2–10 μm
Fine botanical powder 80–120 μm
Standard herbal extract 200–400 μm
Conventional ground material 500+ μm
Scale is logarithmic for clarity. UFP500 targets the sub-10μm range across all ingredient categories — consistent with pharmaceutical fine-particle API standards.
Bioavailability mechanisms

How ultra-fine particle processing changes absorption.

UFP500° particle reduction does not improve bioavailability through a single mechanism — it affects several simultaneously. Understanding the distinct contributions matters for predicting which ingredient categories will respond most significantly.

01

Cell wall liberation

Most of what plants contain never actually gets out.

At conventional particle sizes, the bioactive compounds in botanical, fungal, and fruit materials are physically locked inside intact cell walls. Only what escapes from cells that happen to rupture during chewing or digestion becomes accessible — a variable, incomplete process that happens differently in every person, every time. UFP500° processing opens cell walls mechanically, at defined particle scale, before ingestion. The compound is liberated under controlled conditions — not left to the unpredictability of the digestive process. This is the upstream step that all other mechanisms depend on: a compound still trapped inside a cell wall cannot dissolve, cannot cross a mucosal surface, and cannot be absorbed. This mechanism is particularly significant for fungal materials, where a chitin-reinforced wall architecture makes conventional digestion especially ineffective at releasing intracellular beta-glucans and polysaccharides. (Holland et al., Foods, 2020; Gil-Chávez et al., Food Chemistry, 2021)

02

Surface area — dissolution kinetics

Smaller particles dissolve faster — and at 1–5 microns, fast enough to not need a carrier.

The Noyes-Whitney equation governs dissolution rate: it is directly proportional to surface area. Reducing particle diameter by 100× increases surface area by 100× in equivalent mass. At 1–5 microns, poorly soluble compounds that would ordinarily require lipid formulation or solubilisation technology dissolve at physiologically relevant rates in aqueous media alone — removing the formulation dependency on emulsifiers, carriers, and excipients. This is particularly significant for hydrophobic polyphenols, terpenoids, and fat-soluble vitamins. A controlled human crossover trial (n=23) demonstrated 5–14× bioavailability uplift from micronisation alone — no carrier, no surfactant, no piperine. (Sasaki et al., Mol Nutr Food Res, 2014)

dC/dt = DA(Cs−C) / hV
03

Preserved thermal-labile bioactivity

Heat destroys the very things that make botanicals work — cold process keeps them intact.

Ultra-fine particle reduction without heat is a prerequisite for the mechanisms that follow. Myrosinase — the enzyme responsible for sulforaphane conversion in broccoli — is denatured above approximately 60°C. Polyphenol oxidation accelerates significantly above 40°C. Volatile terpene compounds are lost at even lower temperatures. Cold-process particle refinement preserves these structures through the full processing cycle — a category of benefit that conventional hot-milling and spray-drying cannot offer. The compound arrives in the formulation as it left the plant.

04

Enzymatic access and bioactivation

Some compounds aren't active until an enzyme converts them — that conversion only works if the surfaces can meet.

For glucosinolate-containing botanical systems — most critically broccoli sprout — the conversion of glucoraphanin to sulforaphane requires intimate contact between substrate and the enzyme myrosinase. At conventional particle sizes, this reaction is incomplete: cells containing glucoraphanin and cells containing myrosinase never fully meet. UFP500° processing increases the contact surface between these compartments, substantially improving conversion yield. Human data illustrates the scale of the difference: raw broccoli (intact myrosinase, high surface contact) delivers 37% sulforaphane bioavailability; cooked broccoli (heat-denatured myrosinase) delivers 3.4% — an 11× gap caused entirely by the same variable UFP500° controls. (Vermeulen et al., J Agric Food Chem, 2008)

Glucoraphanin + Myrosinase → Sulforaphane + Glucose
05

Mucosal contact and transit dynamics

Smaller particles stay in the right place in the gut long enough to actually be absorbed.

Particles in the 1–10 micron range spend more time in proximity to the intestinal epithelial surface than larger particles, which settle faster and disperse less completely in luminal fluid. This increases the concentration gradient driving passive diffusion, and prolongs the residence time of active compounds in the absorptive zone of the small intestine — the narrow window where most absorption actually occurs.

06

M-cell and lymphatic uptake

There's a backdoor into the bloodstream that bypasses the liver — and it's selective for particles exactly this size.

Peyer's patches and associated M-cells in the gut-associated lymphoid tissue can sample and transcytose particles in the 1–5 micron range. Unlike conventional intestinal absorption — which routes compounds through the portal vein and then the liver, where many botanical compounds are significantly metabolised — particles absorbed via Peyer's patches enter lymphatic circulation directly, bypassing hepatic first-pass metabolism. This is particularly relevant for lipophilic botanical compounds — terpenoids, fat-soluble polyphenols, resin acids — where liver metabolism would otherwise substantially reduce systemic exposure. The particle size sensitivity of this mechanism is well-documented in the oral vaccine delivery literature.

Material categories

The question is not what. It is how — and what happens next.

UFP500 processes natural materials across botanical, fungal, marine, cereal, and fruit categories on a continuous basis. What matters is not a list of ingredients — it is understanding what ultra-fine particle processing unlocks in each material class, and why that changes what is possible in the application it is destined for.

Botanical & herbal

Aromatic herbs, resins, roots, and plant-derived bioactive fractions. Ultra-fine particle processing dramatically improves the accessibility of polyphenols, terpenoids, and volatile compounds that conventional processing leaves largely trapped inside plant cell wall structures.

Fruit & pomace

Fresh, dried, and upcycled fruit fractions — including juice and processing industry side streams with significant residual bioactive value. Anthocyanins, ellagitannins, and carotenoid fractions respond strongly to ultra-fine particle processing, with cold-process preservation of oxidation-sensitive pigment fractions critical to outcome quality.

Cereal & grain

Malt, whole grain fractions, and cereal-derived enzyme systems. Ultra-fine particle processing changes how these materials behave in dough matrices, fermentation systems, and functional food formats — opening new textural, nutritional, and bioactive dimensions that conventional milling cannot reach.

Marine & coastal biomass

Crustacean shell biomass, microalgae, and coastal botanical matrices — among the most structurally complex and underutilised bioactive sources available. Ultra-fine particle processing unlocks chitin fractions, marine polyphenols, and structurally complex carbohydrates that standard processing cannot efficiently access.

Fungal & mycological

Medicinal and functional fungi with complex cell wall architecture that severely limits bioavailability in conventionally processed form. Ultra-fine particle processing disrupts cell walls mechanically — without heat or solvents — releasing beta-glucan, polysaccharide, and bioactive alkaloid fractions into a form the body can actually use.

Novel & emerging

UFP500 processes new materials on a continuous basis. The platform expands with every material that moves through it. If the scientific rationale is clear and the application context is a genuine fit, the conversation starts with a feasibility evaluation — not a capability checklist.

UFP500° distinction mark

The mark that tells the story.

UFP500° appears on products built on the ultra-fine particle processing standard documented on this site — cold-process, solvent-free, additive-free, with particle size verified by laser diffraction per reference material. The mark speaks to performance, natural integrity, and clean-label output simultaneously. The science here is what it promises.

Visit ufp500.com
Frequently asked

Questions scientists ask first.

Answers to the questions that come up in every early conversation — honest, direct, and without regulatory overreach.

No. UFP500 produces particles in the 1–5 μm range (D50), with D10 below 1 μm and D90 below 10 μm. Nanotechnology is generally defined as particles below 100 nanometres (0.1 μm). UFP500-processed material sits well above that threshold and does not carry the regulatory, toxicological, or safety considerations associated with true nanoparticles. This is an important distinction — and one we take seriously.

Particle size distribution is measured by laser diffraction — the standard analytical method for this size range — and confirmed by scanning electron microscopy (SEM) where morphology matters. D10, D50, and D90 values are documented for every reference material. Results are ingredient-specific and vary based on feedstock characteristics and application target.

UFP500 operates as a cold process — no heat is applied, and ambient temperature is maintained throughout. This is critical for enzymatically active materials (myrosinase is denatured above ~60°C), polyphenol-rich fractions (oxidation accelerates with heat), and volatile terpene compounds. Bioactive retention is assessed against incoming material reference values for each ingredient category processed.

No solvents. No additives. No carrier agents. UFP500 is a dry, mechanical process. The output is the input ingredient — at a fundamentally different particle scale. This clean-label characteristic is one of the platform's most commercially relevant properties, particularly for food, supplement, and veterinary applications where label simplicity matters.

It refers to the potential uplift in bioavailable fraction relative to the same ingredient processed conventionally — based on the surface area increase achieved at 1–5 μm particle scale (50–100× surface area increase drives substantially faster dissolution and improved absorption kinetics). The actual improvement observed in any specific application depends on the ingredient, the matrix, the delivery format, and the absorption pathway involved. We report what the physics predicts and what our materials demonstrate — not a universal statement. Application-specific validation is always the right next step.

No — and this is important. Particle size is a proxy for performance, not the end goal. What matters is how the processed material behaves in its intended application: dissolution rate in a liquid matrix, dispersion in a dough, absorption across a mucosal surface, enzymatic activity in a biological system. The optimal particle size for any given application is defined by the science of that application. UFP500 processing parameters are set accordingly — and validated through the application itself, not through particle size alone.

No. UFP500 is a processing platform — it improves how ingredients behave. Health and medical claims are the domain of the specific formulation, the application, and the regulatory framework governing that product in its market. BioFund's own research programmes (SENTINEL, companion animal oncology, circular bioeconomy) generate data that informs those claims through proper scientific process. We describe what the physics does. The biology is for the science to determine.

Research context

UFP500 within BioFund's science.

UFP500 is not an isolated processing technology — it is the operational foundation of BioFund's botanical and nutraceutical research pipeline. The science informing the platform is the same science driving BioFund's therapeutic development work.

OneHealth — the framework

Companion animals develop the same diseases.

Dogs and cats develop spontaneous cancers, osteoarthritis, inflammatory bowel disease, cognitive dysfunction, and metabolic disease with the same underlying biology as their human companions — often in comparable prevalence, in shared environments, exposed to many of the same dietary and environmental pressures. This shared biology is why BioFund's OneHealth methodology treats companion animal health as a research destination in its own right. Oral absorption pharmacokinetics in dogs are well-documented and directly comparable to human data across many compound classes — making them the most scientifically credible, ethically aligned non-rodent model available for botanical bioavailability science. UFP500-processed materials are the formulation core of this pipeline.

Circular bioeconomy

Upcycled ingredient streams

Industrial food and aquaculture processing generates enormous volumes of side streams — crustacean shell biomass, fruit and vegetable pomace, grain fractions, seed press cakes — that conventional technology cannot valorize into anything beyond low-grade disposal. UFP500 changes that equation. Ultra-fine particle processing unlocks the bioactive and structural compounds locked inside these complex matrices — chitin fractions, marine polyphenols, carotenoids, enzyme systems — transforming an industry disposal problem into a premium ingredient platform. The environmental and economic logic is the same: the most valuable material is the one already produced.

SENTINEL programme

Occupational exposure & functional nutrition

Professional firefighters carry an IARC Group 1 occupational carcinogen classification — a burden that BioFund believes nutritional science has a meaningful role in addressing. SENTINEL is a long-term development commitment to that science. UFP500-processed botanicals are the formulation core of a cold-formed functional nutrition concept targeting combustion-exposure biomarkers. The formulation follows the science.

UFP Atlas — Material Science

Systematic characterisation across the material library

Every material processed through UFP500 infrastructure generates a structured dataset — particle size distribution (D10, D50, D90), bioactive retention against incoming reference values, dissolution kinetics, and application-specific behaviour documentation. This body of material science, accumulated across botanical, fungal, marine, cereal, and fruit categories, is what the UFP Atlas is built from. Not a product catalogue — a living scientific dataset that makes feasibility evaluation faster, cross-material learning computable, and the platform's depth verifiable.

Literature & Evidence

The science behind the science.

Every mechanism and material statement on this site is grounded in peer-reviewed literature. The references below are organized by topic. A full independent fact-check of all ufp500.org content is available as a linked document.

Dissolution Physics & Surface Area
Noyes, A.A. & Whitney, W.R. The Rate of Solution of Solid Substances in Their Own Solutions J Am Chem Soc, 19(12):930–934 · 1897 doi.org/10.1021/ja02086a003
Various. Dissolution Kinetics of a BCS Class II Active Pharmaceutical Ingredient — particle size and surface area as rate determinants Crystal Growth & Design · PMC8014923 · 2021 PMC8014923
Malvern Panalytical. Basic Principles of Particle Size Analysis — surface area and the sphere model Technical Reference · Malvern Panalytical malvernpanalytical.com
Bioavailability Enhancement — Human Data
Sasaki H. et al. Innovative preparation of curcumin for improved oral bioavailability — micronised powder delivers 5–14× uplift vs. native powder, no excipients (n=23, crossover RCT) Mol Nutr Food Res, 58(3):516–527 · 2014 · NCT01925287 doi.org/10.1002/mnfr.201300724
Sun J. et al. Effect of particle size on solubility, dissolution rate, and oral bioavailability — CoQ10, 4.4× AUC uplift from particle size reduction alone, in vivo Int J Nanomedicine, 7:5733–5744 · 2012 PubMed 23166438
Meins J. et al. Enhanced absorption of boswellic acids — AKBA produces 56× AUC increase vs. native resin with micellar solubilisation J Pharm Pharmacol · 2018 Mendeley record
Mucosal Contact & Absorption Dynamics
Wilson F.A. et al. Unstirred Water Layers in Intestine: Rate Determinant of Fatty Acid Absorption from Micellar Solutions Science, 174(4013):1031–1033 · 1971 doi.org/10.1126/science.174.4013.1031
Sugano K. Possible reduction of effective thickness of intestinal unstirred water layer by particle drifting effect Int J Pharmaceutics, 387(1–2):103–109 · 2010 doi.org/10.1016/j.ijpharm.2009.12.012
M-cell, GALT & Lymphatic Transport
Knoop K.A. & Newberry R.D. Unique insights into the intestinal absorption, transit, and subsequent biodistribution of polymer-derived microspheres (500 nm–5 μm) PNAS, 110(34):13803–13808 · 2013 doi.org/10.1073/pnas.1305882110
Beier R.C. & Gebert U. Kinetics of particle uptake in the domes of Peyer's patches — transcytosis of 3.4 μm yeast particles by M-cells confirmed within <1 hour Am J Physiol · PubMed 9655693 · 1998 PubMed 9655693
EFSA Scientific Committee. Intestinal Uptake of Particles — particles <10 μm taken up by M-cells into GALT; lymphatic transport to systemic circulation confirmed EFSA Technical Report · European Food Safety Authority efsa.europa.eu (PDF)
StatPearls / NCBI Bookshelf. First-Pass Effect — pharmacological basis for lymphatic transport bypass of hepatic first-pass metabolism via oral particulate absorption NCBI Bookshelf · NBK551679 NBK551679
Enzymatic Mechanisms — Myrosinase & Sulforaphane
Vermeulen M. et al. Bioavailability and kinetics of sulforaphane in humans after consumption of cooked versus raw broccoli — raw: 37%; cooked: 3.4% (n=8, human controlled crossover trial) J Agric Food Chem, 56(22):10505–10509 · 2008 doi.org/10.1021/jf801989e
Fahey J.W. et al. Exogenous myrosinase doubles sulforaphane bioavailability in humans — 39.8% ± 3.1% with myrosinase vs. 18.6% ± 3.1% without (randomised double-blind crossover) Sci Rep · 2026 PMC4629881
Rungapamestry V. et al. Thermosonication and myrosinase activity — thermal treatment at 50°C reduces myrosinase activity 14.9–23.8%; at 60°C, 35.4–59.3% PMC7923798 · 2021 PMC7923798
Cell Wall Architecture & Liberation
Holland C. et al. Plant Cell Walls: Impact on Nutrient Bioaccessibility and Digestibility — cell walls confirmed as primary physical barrier to intracellular bioactive release during digestion Foods · PMC7074226 · 2020 PMC7074226
Gil-Chávez G.J. et al. Polyphenols are naturally located within plant cells and do not contact the cell wall surface prior to processing — conventional digestion releases only the fraction from disrupted cells Food Chemistry · ScienceDirect · 2021 ScienceDirect
Various. Engineering Strategies for Fungal Cell Disruption — chitin-reinforced cell wall as primary barrier to beta-glucan access; cold mechanical disruption preserves structural integrity Analytical Science Journals · 2025 Analytical Science Journals
Thermal Sensitivity — Polyphenols & Terpenes
Various. Recent Advances of Polyphenol Oxidases in Plants — PPO optimum temperature 15–50°C; oxidation accelerates significantly above this range PMC10004730 · 2023 PMC10004730
Various. Effect of drying temperature on terpene retention — total terpene retention decreases from 82.1% to 29.9% as temperature rises from ambient to 90°C ScienceDirect · 2021 ScienceDirect
Particle Measurement & Nanotechnology Definition
European Commission. Commission Recommendation 2011/696/EU — nanomaterial definition: 1–100 nm. UFP500 at D50 1–5 μm is not a nanomaterial under EU or ISO definitions Official Journal of the EU · 2011 EUR-Lex
Malvern Panalytical. D90, D50, D10 — volume-weighted percentile diameters; ISO 13320 standard for laser diffraction particle size analysis Technical Reference · Malvern Panalytical malvernpanalytical.com
Occupational Health — Firefighters
IARC Working Group. IARC Monographs Volume 132: Occupational Exposure as a Firefighter — Group 1 (carcinogenic to humans); sufficient evidence for mesothelioma and bladder cancer IARC Monographs · Lyon · 2022 iarc.who.int
Independent verification

A systematic independent fact-check of all ufp500.org content is available as a complete reference document.

Full science review →